The second monoclonal antibodies was indeed bought regarding Pharmingen (North park, Calif
The second monoclonal antibodies was indeed bought regarding Pharmingen (North park, Calif Cellphone staining and you can move cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaNstep three) …